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Mutants with defined insertions confirmed to cause mutant phenotypes in the PML collection

Mutant lines are available on request from us, or from the Maize Genetics Cooperative

Gene	# of Alleles	Genbank Accession	Rice Ortholog	Arabidopsis Ortholog	POG	Gene Function
caf1	2	Q84N49	Os01g31110	AT2G20020	7461	CRS2-associated factor 1; chloroplast group II intron splicing
caf2	1	Q84N48	Os01g21990	AT1G23400	2465	CRS2-associated factor 2; chloroplast group II intron splicing
crp1	7	AAC25599	Os07g36390	AT5G42310	19380	Chloroplast PPR protein: Activates petA and psaC translation; stabilizes processed petB and petD mRNAs
crs1	6	Q9FYT6	Os08g27150	AT5G16180	18231	Chloroplast RNA Splicing 1; promotes chloroplast atpF intron splicing
crs2	2	Q9M5P4	Os01g04130	AT5G16140, AT5G38290	18225	Chloroplast RNA Splicing 2; required for splicing of chloroplast subgroup IIB introns
csy1	2	O63066	Os08g15460	AT2G18710	7343	CpSecY: thylakoid protein translocase
ppr2	2	AAP37977	Os03g19650	AT3G06430	10729	Chloroplast PPR protein: Required for expression of one or more chloroplast gene that functions in chloroplast translation
ppr4	2	ABF57644	Os04g58780	AT5G04810	17392	Chloroplast PPR protein: required for rps12 trans-splicing
ppr5	2	ABS82814	Os02g51480	AT4G39620	17115	Stabilizes unspliced trnG precursor RNA in chloroplast; may promote trnG splicing
crs4	2		Os06g09880	AT3G18110	11746	Chloroplast RNA splicing 4: a PPR protein required for ndhA, ndhB splicing; aka ppr7
ppr10	2	ACL01094	Os05g19380	AT2G18940	7369	Chloroplast PPR protein: stabilizes specific processed chloroplast RNAs
ppr100*	2		Os02g02590	AT2G01860	26034	Chloroplast PPR protein: required for biogenesis of plastid translation machinery
ppr103*	3		Os07g07620	AT5G03800	17332	Chloroplast PPR protein: required for biogenesis of plastid translation machinery
ZmOtp51*	2		Os02g47360	AT2G15820	7081	Chloroplast PPR protein: required for ycf3-2 intron splicing
pprP67*	4		Os03g11670	AT4G16390	15339	Chloroplast PPR protein; mutants have pale green leaves. Molecular function unkown
rnc1	4	ABR53724	Os01g59510	AT4G37510	16993	Chloroplast RNAse III domain protein: required for the splicing of many chloroplast introns
why1	2	ACC60344	Os0605350	AT2G02740, AT1G14410	1521	Chloroplast single-stranded RNA and DNA binding protein. Required for chloroplast biogenesis
vdl1	2		Os01g73900	AT5G19210	18431	Chloroplast DEAD box protein; Ortholog of tobacco “VDL helicase”. Required for biogenesis of plastid translation machinery
cpx1*	2					Mutant phenotype: necrotic seedlings
ZmCHLH*	2		Os03g20700	AT5G13630	30887	Chlorophyll synthesis: magnesium-chelatase subunit H; aka ZmGUN5
tha1	1		Os01g21820	AT4G01800	14450	Chloroplast SecA homolog: required for thylakoid protein targeting
tha4	1	AAD31522	Os03g43430	AT5G28750	18981	Thylakoid protein targeting: tat pathway
tha8	6	AAS55479	Os01g06480	AT3G27750	12508	Mutant has defect in tat thylakoid targeting pathway; biochemical function unknown
csr1*	3	CAD70570	Os01g72800	AT2G45770	9886	CpFtsY: chloroplast SRP receptor. Involved in thylakoid protein targeting
wtf1	3	ACI96105	Os05g49610	AT4G01037	39488	Chloroplast DUF860 protein: RNA binding protein required for chloroplast RNA splicing

* Mutant alleles recovered in reverse-genetic screens in collaboration with outside users.

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